Analysis Note

Negative ControlMMP-9 protein (Cat. Nos. PF024 or PF038)

Positive ControlMMP-2 protein (Cat. Nos. PF023 or PF037)

Application

Frozen Sections (see application references)Immunoblotting (1 g/ml)Paraffin Sections (see application references)

General description

Recognizes the ~72 kDa latent and the ~66 kDa active forms of the MMP-2.

This Anti-MMP-2 (Ab-3) Mouse mAb (42-5D11) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-2 (Ab-3).

Purified mouse monoclonal antibody (see application references). Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.

Immunogen

a synthetic peptide (VTPRDKPMGPLLVATF) corresponding to amino acids 468-483 of human MMP-2

Epitope: within amino acids 468-483 of human MMP-2 (numbered from the propeptide)

Human

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody will not cross-react with human MMP-1, MMP-3, MMP-9, or MMP-13. Antibody should be titrated for optimal results in individual systems.

Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol.2, 861.Fujimoto, N., et al. 1993. Clinica Chimica Acta.221, 91.Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.Woessner, J. F. 1991. FASEB J.5, 2145.Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)